DNA mini-barcodes in taxonomic assignment: a morphologically unique new homoneurous moth clade from the Indian Himalayas described in Micropterix (Lepidoptera, Micropterigidae)

642 a 2010 Lees, D. C., Rougerie, R., Zeller-Lukashort, C. & Kristensen, N. P. (2010). DNA minibarcodes in taxonomic assignment: a morphologically unique new homoneurous moth clade from the Indian Himalayas described in Micropterix (Lepidoptera, Micropterigidae). — Zoologica Scripta, 39, 642–661. The first micropterigid moths recorded from the Himalayas, Micropterix cornuella sp. n. and Micropterix longicornuella sp. n. (collected, respectively, in 1935 in the Arunachel Pradesh Province and in 1874 in Darjeeling, both Northeastern India) constitute a new clade, which is unique within the family because of striking specializations of the female postabdomen: tergum VIII ventral plate forming a continuous sclerotized ring, segment IX bearing a pair of strongly sclerotized lateroventral plates, each with a prominent horn-like posterior process. Fore wing vein R unforked, all Rs veins preapical; hind wing devoid of a discrete vein R. The combination of the two first-mentioned vein characters suggests close affinity to the large Palearctic genus Micropterix (to some species of which the members of the new clade bear strong superficial resemblance). Whilst absence of the hind wing R is unknown in that genus, this specialization is not incompatible with the new clade being subordinate within it. A 136-bp fragment of Cytochrome oxidase I successfully amplified from both of the 75-year-old specimens strongly supports this generic assignment. Translated to amino acids, this DNA fragment is highly diagnostic of this genus, being identical to that of most (16 of the 26) Micropterix species studied comparatively here, 1–4 codons different from nine other species (including Micropterix wockei that in phylogenetic analyses we infer to be sister to other examined species), whilst 7–15 codons different to other amphiesmenopteran genera examined here. A dating analysis also suggests that the large clade excluding M. wockei to which M. cornuella belongs appeared <31 million years ago. These findings encourage discovery of a significant radiation of Micropterix in the Himalayan region. Our analysis has more general implications for testing the assignment of DNA mini-barcodes to a taxon, in cases such as museum specimens where the full DNA barcode cannot be recovered. Corresponding author: David C. Lees, Centre de Recherche d’Orléans, INRA, Orléans 2163, Avenue de la Pomme de Pin, CS 45001 Ardon, 45075 Orléans Cedex 2, France. E-mails: [email protected], [email protected], [email protected] David C. Lees, Natural History Museum, London SW7 5BD, UK; Centre de Recherche d’ Orléans, INRA, UR 633 Zoologie Forestière, 45075 Orléans, France Rodolphe Rougerie, Canadian Centre for DNA Barcoding, Biodiversity Institute of Ontario, 50 Stone Road East, Guelph, Ontario, N1G 2W1 Canada. E-mail: [email protected] Christof Zeller-Lukashort, Forsthubfeld 14, 5303 Thalgau, Austria. E-mail: christof.zeller@ gmx.net Niels P. Kristensen, Natural History Museum of Denmark (Zoological Museum), University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen Ø, Denmark. E-mail: npkristensen@ snm.ku.dk